Structural basis for aminoglycoside inhibition of bacterial ribosome recycling

@article{Borovinskaya2007StructuralBF,
  title={Structural basis for aminoglycoside inhibition of bacterial ribosome recycling},
  author={M. A. Borovinskaya and R. Pai and W. Zhang and B. S. Schuwirth and J. Holton and G. Hirokawa and H. Kaji and A. Kaji and J. Cate},
  journal={Nature Structural &Molecular Biology},
  year={2007},
  volume={14},
  pages={727-732}
}
  • M. A. Borovinskaya, R. Pai, +6 authors J. Cate
  • Published 2007
  • Biology, Medicine
  • Nature Structural &Molecular Biology
  • Aminoglycosides are widely used antibiotics that cause messenger RNA decoding errors, block mRNA and transfer RNA translocation, and inhibit ribosome recycling. Ribosome recycling follows the termination of protein synthesis and is aided by ribosome recycling factor (RRF) in bacteria. The molecular mechanism by which aminoglycosides inhibit ribosome recycling is unknown. Here we show in X-ray crystal structures of the Escherichia coli 70S ribosome that RRF binding causes RNA helix H69 of the… CONTINUE READING
    273 Citations

    Figures, Tables, and Topics from this paper

    Allosteric control of the ribosome by small-molecule antibiotics
    • 104
    Structural Basis for Ribosome Recycling by RRF and tRNA
    • 6
    Bacterial Protein Synthesis as a Target for Antibiotic Inhibition.
    • 42
    • Highly Influenced
    • PDF
    Structural Insights into ribosome recycling factor interactions with the 70S ribosome.
    • 58
    Impairment of ribosomal subunit synthesis in aminoglycoside-treated ribonuclease mutants of Escherichia coli
    • 12
    Pseudouridine modifications influence binding of aminoglycosides to helix 69 of bacterial ribosomes.
    • 2
    • PDF
    Aminoglycoside interactions and impacts on the eukaryotic ribosome
    • 54
    • Highly Influenced
    • PDF

    References

    SHOWING 1-10 OF 53 REFERENCES
    Structural origins of gentamicin antibiotic action
    • 300
    • PDF
    Visualization of ribosome-recycling factor on the Escherichia coli 70S ribosome: Functional implications
    • R. Agrawal, M. Sharma, +6 authors J. Frank
    • Biology, Medicine
    • Proceedings of the National Academy of Sciences of the United States of America
    • 2004
    • 147
    • PDF
    Evidence for in vivo ribosome recycling, the fourth step in protein biosynthesis
    • 127
    • PDF
    Intersubunit movement is required for ribosomal translocation
    • 129
    • PDF
    Structure of the 70S Ribosome Complexed with mRNA and tRNA
    • 1,216
    • PDF
    Inhibition of ribosomal translocation by aminoglycoside antibiotics.
    • 101