Structural basis for Pan3 binding to Pan2 and its function in mRNA recruitment and deadenylation

@inproceedings{Wolf2014StructuralBF,
  title={Structural basis for Pan3 binding to Pan2 and its function in mRNA recruitment and deadenylation},
  author={Jana Wolf and Eugene Valkov and Mark D. Allen and Birthe Meineke and Yuliya Gordiyenko and Stephen H McLaughlin and Tayla M. Olsen and Carol V Robinson and Mark Bycroft and Murray Stewart and Lori A Passmore},
  booktitle={The EMBO journal},
  year={2014}
}
The conserved eukaryotic Pan2-Pan3 deadenylation complex shortens cytoplasmic mRNA 3' polyA tails to regulate mRNA stability. Although the exonuclease activity resides in Pan2, efficient deadenylation requires Pan3. The mechanistic role of Pan3 is unclear. Here, we show that Pan3 binds RNA directly both through its pseudokinase/C-terminal domain and via an N-terminal zinc finger that binds polyA RNA specifically. In contrast, isolated Pan2 is unable to bind RNA. Pan3 binds to the region of Pan2… CONTINUE READING
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