Structural basis for DNA duplex separation by a superfamily-2 helicase

Abstract

To reveal the mechanism of processive strand separation by superfamily-2 (SF2) 3′→5′ helicases, we determined apo and DNA-bound crystal structures of archaeal Hel308, a helicase that unwinds lagging strands and is related to human DNA polymerase θ. Our structure captures the duplex-unwinding reaction, shows that initial strand separation does not require… (More)
DOI: 10.1038/nsmb1246

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