Structural basis for CRISPR RNA-guided DNA recognition by Cascade

  title={Structural basis for CRISPR RNA-guided DNA recognition by Cascade},
  author={M. M. Jore and M. Lundgren and E. Duijn and J. B. Bultema and E. Westra and S. Waghmare and B. Wiedenheft and {\"U}mit Pul and R. Wurm and R. Wagner and M. R. Beijer and A. Barendregt and K. Zhou and A. L. Snijders and M. Dickman and J. Doudna and E. Boekema and A. Heck and J. Oost and Stan J J Brouns},
  journal={Nature Structural &Molecular Biology},
  • M. M. Jore, M. Lundgren, +17 authors Stan J J Brouns
  • Published 2011
  • Biology, Medicine
  • Nature Structural &Molecular Biology
  • The CRISPR (clustered regularly interspaced short palindromic repeats) immune system in prokaryotes uses small guide RNAs to neutralize invading viruses and plasmids. In Escherichia coli, immunity depends on a ribonucleoprotein complex called Cascade. Here we present the composition and low-resolution structure of Cascade and show how it recognizes double-stranded DNA (dsDNA) targets in a sequence-specific manner. Cascade is a 405-kDa complex comprising five functionally essential CRISPR… CONTINUE READING
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