Structural and ultrastructural analysis of Solanum lycopersicoides protoplasts during diploid plant regeneration.

@article{Tylicki2002StructuralAU,
  title={Structural and ultrastructural analysis of Solanum lycopersicoides protoplasts during diploid plant regeneration.},
  author={Adam Tylicki and Wojciech Burza and Stefan Malepszy and Mariola Kulawiec and Mieczysław Kuraś},
  journal={Annals of botany},
  year={2002},
  volume={90 2},
  pages={269-78}
}
Light, fluorescence and electron microscopy were used to analyse the structural properties of protoplasts obtained from established suspension culture of Solanum lycopersicoides Dun, composed of meristematic cell aggregates. Four types of protoplasts were distinguished immediately after isolation: (1) mononuclear; (2) polynuclear, (3) anuclear and (4) homogeneous protoplasts. Only mononuclear protoplasts were capable of complete cell wall regeneration and mitotic division. Other types of… CONTINUE READING