Structural and mutagenetic analyses of a 1,3-1,4-β-glucanase from Paecilomyces thermophila.

Abstract

The thermostable 1,3-1,4-β-glucanase PtLic16A from the fungus Paecilomyces thermophila catalyzes stringent hydrolysis of barley β-glucan and lichenan with an outstanding efficiency and has great potential for broad industrial applications. Here, we report the crystal structures of PtLic16A and an inactive mutant E113A in ligand-free form and in complex with… (More)
DOI: 10.1016/j.bbapap.2013.11.005

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