Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution.


Galactose oxidase (GO; E.C. is a copper- containing enzyme that oxidizes a range of primary alcohols to aldehydes. This broad substrate specificity is reflected in a high K(M) for substrates. Directed evolution has previously been used to select variants of GO that exhibit enhanced expression and kinetic properties. In assays using unpurified… (More)


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