Structural and kinetic insight into the biosynthesis of H2S and L-lanthionine from L-cysteine by a PLP-dependent enzyme from Fusobacterium nucleatum.

@article{Mothersole2019StructuralAK,
  title={Structural and kinetic insight into the biosynthesis of H2S and L-lanthionine from L-cysteine by a PLP-dependent enzyme from Fusobacterium nucleatum.},
  author={Robert G Mothersole and K. Wolthers},
  journal={Biochemistry},
  year={2019}
}
Fusobacterium nucleatum is a common oral bacterium and a major producer of H2S, a toxic gas linked to the pathogenesis of periodontal disease. The bacterium encodes a fold type II pyridoxal-L-phosphate (PLP)-dependent enzyme, Fn1220 or lanthionine synthase (LS) that generates H2S and L-lanthionine (a component of the peptidoglycan layer) through β-replacement of L-cysteine by a second molecule of L-cysteine. Herein, we show through detailed kinetic analysis that LS elicits catalytic promiscuity… Expand