Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata.

  title={Structural and functional study of an Anemonia elastase inhibitor, a "nonclassical" Kazal-type inhibitor from Anemonia sulcata.},
  author={H. Hemmi and T. Kumazaki and K. Yoshizawa-Kumagaye and Y. Nishiuchi and Takuya Yoshida and T. Ohkubo and Yuji Kobayashi},
  volume={44 28},
Anemonia elastase inhibitor (AEI) is a "nonclassical" Kazal-type elastase inhibitor from Anemonia sulcata. Unlike many nonclassical inhibitors, AEI does not have a cystine-stabilized alpha-helical (CSH) motif in the sequence. We chemically synthesized AEI and determined its three-dimensional solution structure by two-dimensional NMR spectroscopy. The resulting structure of AEI was characterized by a central alpha-helix and a three-stranded antiparallel beta-sheet of a typical Kazal-type… Expand
Characterization and comparative 3D modeling of CmPI-II, a novel ‘non-classical’ Kazal-type inhibitor from the marine snail Cenchritis muricatus (Mollusca)
A 3D model of the CmPI-II/HNE complex provides a structural framework for the interpretation of its experimentally determined K i value and may define a new group of ‘non-classical’ Kazal inhibitors according to its CysI-CysV disulfide bridge position. Expand
NMR structure of CmPI-II, a non-classical Kazal protease inhibitor: Understanding its conformational dynamics and subtilisin A inhibition.
The 3D structure obtained provides the structural determinants for the subtilisin A inhibition by CmPI-II and allows the designing of more specific and potent molecules. Expand
Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum
A kazal-type serine protease inhibitor named ShSPI was identified from the cDNA library of the venom glands of Scolopendra hainanum and showed significant inhibitory effects on porcine pancreatic elastase and human neutrophils elastases. Expand
Crystal structure of greglin, a novel non‐classical Kazal inhibitor, in complex with subtilisin
It is shown that greglin resists denaturation at high temperature (95 °C) and after exposure to acetonitrile and acidic or basic pH, and it is proposed that the C‐terminal region is responsible for non‐favourable interactions with the autolysis loop of serine proteases of the chymotrypsin family, and thus governs specificity. Expand
Crystallization of a nonclassical Kazal-type Carcinoscorpius rotundicauda serine protease inhibitor, CrSPI-1, complexed with subtilisin.
The serine protease inhibitor CrSPI isoform 1 (CrSPI-1), a unique nonclassical Kazal-type inhibitor of molecular weight 9.3 kDa, was identified from the hepatopancreas of the horseshoe crab Carcinoscorpius rotundicauda and potently inhibits subtilisin and constitutes a powerful innate immune defence against invading microbes. Expand
Arginine substitution by alanine at the P1 position increases the selectivity of CmPI-II, a non-classical Kazal inhibitor
The alanine substitution at P1 site on CmPI-II abolishes the trypsin inhibition, confirming the relevance of an arginine residue at P 1 site in Cm PI-II for trypsIn inhibition and leading to a molecule with more potentialities in biomedicine. Expand
A novel locust (Schistocerca gregaria) serine protease inhibitor with a high affinity for neutrophil elastase.
The purified to homogeneity two forms of a new serine protease inhibitor specific for elastase/chymotrypsin from the ovary gland of the desert locust Schistocerca gregaria are purified and suggest that it can be used as a template to design a new generation of highly resistantElastase inhibitors for treating inflammatory diseases. Expand
Structural Basis for Dual-Inhibition Mechanism of a Non-Classical Kazal-Type Serine Protease Inhibitor from Horseshoe Crab in Complex with Subtilisin
Elucidation of the structure of the CrSPI-1: subtilisin (1∶2) ternary complex increases the understanding of host-pathogen interactions in the innate immune system at the molecular level and provides new strategies for immunomodulation. Expand
A Kazal-Type Serine Protease Inhibitor from the Defense Gland Secretion of the Subterranean Termite Coptotermes formosanus Shiraki
The structure and function of one of several proteins found in the termite defense gland secretion is reported, which represents the first structure from the taxonomic family, Rhinotermitidae. Expand
Purification and partial characterization of human neutrophil elastase inhibitors from the marine snail Cenchritis muricatus (Mollusca).
  • Y. González, A. Tanaka, +4 authors M. Chavez
  • Chemistry, Medicine
  • Comparative biochemistry and physiology. Part A, Molecular & integrative physiology
  • 2007
Cm PI-I and CmPI-II are the first human neutrophil elastase inhibitors described in a mollusk. Expand