Structural and functional roles of modules in hemoglobin. Substitution of module M4 in hemoglobin subunits.

@article{Inaba1997StructuralAF,
  title={Structural and functional roles of modules in hemoglobin. Substitution of module M4 in hemoglobin subunits.},
  author={Kayo Inaba and Keisuke Wakasugi and Koichiro Ishimori and Takashi Konno and Mikio Kataoka and Isao Morishima},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 48},
  pages={30054-60}
}
The alpha- and beta-subunits of human hemoglobin consist of the modules M1, M2 + M3, and M4, which correspond to the exons 1, 2, and 3, respectively (Go, M. (1981) Nature 291, 90-92). To gain further insight into functional and structural significance of the modules, we designed two kinds of chimeric hemoglobin subunits (chimeric alphaalphabeta- and betabetaalpha-subunits), in which the module M4 was replaced by the partner subunits. CD spectra in the far-UV region showed that the secondary… CONTINUE READING