Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.

@article{Cohen2008StructuralAF,
  title={Structural and functional dissection of Mif2p, a conserved DNA-binding kinetochore protein.},
  author={Randi L. Cohen and Christopher W. Espelin and Peter De Wulf and Peter K. Sorger and Stephen C Harrison and Kim T. Simons},
  journal={Molecular biology of the cell},
  year={2008},
  volume={19 10},
  pages={
          4480-91
        }
}
Mif2p is the budding-yeast orthologue of the mammalian centromere-binding protein CENP-C. We have mapped domains of Saccharomyces cerevisiae Mif2p and studied the phenotyptic consequences of their deletion. Using chromatin immunoprecipitation (ChIP) and electrophoretic mobility shift assays, we have further shown that Mif2p binds in the CDEIII region of the budding-yeast centromere, probably in close spatial association with Ndc10p. Moreover, ChIP experiments show that Mif2p recruits to yeast… CONTINUE READING

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