Structural and functional analysis of a human 7 S K RNA gene.

@article{Krueger1987StructuralAF,
  title={Structural and functional analysis of a human 7 S K RNA gene.},
  author={Warren Krueger and B J. Benecke},
  journal={Journal of molecular biology},
  year={1987},
  volume={195 1},
  pages={31-41}
}
Using purified RNA from HeLa cells, we have synthesized and cloned a cDNA encoding an almost entire 7 S K RNA. This cDNA probe was used to isolate 7 S K RNA gene sequences from a human genomic library by high-stringency colony hybridization. In order to differentiate between functional genes and related sequences, we have used a rapid in-vitro transcription assay of purified phage DNA. With this additional screening criterion applied to selected clones, we have obtained one recombinant phage… CONTINUE READING

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