Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.

  title={Structural and biochemical insights to the role of the CCR4-NOT complex and DDX6 ATPase in microRNA repression.},
  author={Hansruedi Mathys and J{\'e}r{\^o}me Basquin and Sevim Ozgur and Mariusz Czarnocki-Cieciura and Fabien Bonneau and Aafke Aartse and Andrzej Dziembowski and Marcin Nowotny and Elena Conti and W. Filipowicz},
  journal={Molecular cell},
  volume={54 5},
MicroRNAs (miRNAs) control gene expression by regulating mRNA translation and stability. The CCR4-NOT complex is a key effector of miRNA function acting downstream of GW182/TNRC6 proteins. We show that miRNA-mediated repression requires the central region of CNOT1, the scaffold protein of CCR4-NOT. A CNOT1 domain interacts with CNOT9, which in turn interacts with the silencing domain of TNRC6 in a tryptophan motif-dependent manner. These interactions are direct, as shown by the structure of a… CONTINUE READING
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