Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.

@article{Ti2011StructuralAB,
  title={Structural and biochemical characterization of two binding sites for nucleation-promoting factor WASp-VCA on Arp2/3 complex.},
  author={Shih-Chieh Ti and Christopher T. Jurgenson and Bradley J. Nolen and Thomas D. Pollard},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2011},
  volume={108 33},
  pages={E463-71}
}
Actin-related protein (Arp) 2/3 complex mediates the formation of actin filament branches during endocytosis and at the leading edge of motile cells. The pathway of branch formation is ambiguous owing to uncertainty regarding the stoichiometry and location of VCA binding sites on Arp2/3 complex. Isothermal titration calorimetry showed that the CA motif from the C terminus of fission yeast WASP (Wsp1p) bound to fission yeast and bovine Arp2/3 complex with a stoichiometry of 2 to 1 and very… CONTINUE READING
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