Structural and Functional Organization of the Gene Encoding the Human Thyrotropin‐Releasing Hormone Receptor

  title={Structural and Functional Organization of the Gene Encoding the Human Thyrotropin‐Releasing Hormone Receptor},
  author={Vilborg Matre and Per Ivar H{\o}vring and Sigurd {\O}rstavik and Eirik Frengen and Edith Rian and Zlatibor M Velickovic and Rosalind P. Murray‐Mcintosh and Kaare M. Gautvik},
  journal={Journal of Neurochemistry},
Abstract : The thyrotropin‐releasing hormone (TRH) receptor (TRHR) is widely distributed throughout the central and peripheral nervous systems. In addition to its role in controlling the synthesis and secretion of thyroid‐stimulating hormone and prolactin from the anterior pituitary, TRH is believed to act as a neurotransmitter as well as a neuromodulator. We have isolated genomic λ and P1‐derived artificial chromosome clones encoding the human TRHR. The gene was found to be 35 kb with three… Expand
Mapping, CDNA Cloning and Tissue Expression of the Porcine Thyrotropin-Releasing Hormone Receptor Gene
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The human neuroendocrine thyrotropin-releasing hormone receptor promoter is activated by the haematopoietic transcription factor c-Myb.
It is reported here that the human TRHR promoter contains 11 putative response elements for the haematopoietic transcription factor c-Myb and is highly Myb-responsive in transfection assays, implying a novel functional link between the neuroendocrine and the immune systems at the level of promoter regulation. Expand
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The expression of thyrotrophin‐releasing hormone receptor 1 messenger ribonucleic acid in human pituitary adenomas
This work aimed to compare the expression levels of TRHR‐1 mRNA among various types of pituitary adenoma and to clarify whether these levels correlate with the degree of pituitsary hormone response to TRH. Expand
POU domain factors in the neuroendocrine system: lessons from developmental biology provide insights into human disease.
The structure of the POU domain has been solved, facilitating the understanding of how these proteins bind to DNA and regulate transcription via complex protein-protein interactions. Expand
Expression of the synaptotagmin I gene is enhanced by binding of the pituitary-specific transcription factor, POU1F1.
Significant evidence is provided that Syt1 is a target gene regulated by POU1F1 in GH(3) pituitary cells, and transfection studies with a SyT1-luciferase reporter gene are consistent with the presence of an internal, POU 1F1-regulated promoter in the Syt 1 gene. Expand
Analysis of thyrotropin-releasing hormone-signaling components in pituitary adenomas of patients with acromegaly.
It is concluded that the TRH-signaling elements examined are, in general, not directly involved in the mechanisms causing paradoxical GH secretion in acromegalic patients. Expand
The TRHR Gene Is Associated with Hypothalamo-Pituitary Sensitivity to Levothyroxine
An association between serum TSH levels and discrete alleles of the TR HR gene in totally thyroidectomized patients under LT4 therapy is demonstrated and the TRHR gene seems to be a determinant of hypothalamo-pituitary sensitivity to LT4. Expand
Disorders of thyrotropin synthesis, secretion, and function.
  • S. Rose
  • Medicine
  • Current opinion in pediatrics
  • 2000
Advances related to thyrotropin during 1999 included better understanding of the genetic basis of pituitary development and genetic advances in identifying clinical entities and their mechanisms andExpand
The syndrome of central hypothyroidism and macroorchidism: IGSF1 controls TRHR and FSHB expression by differential modulation of pituitary TGFβ and Activin pathways
Two relevant molecular mechanisms linked to central hypothyroidism and macroorchidism in I GSF1 deficiency are identified, revealing IGSF1 as an important regulator of TGFβ/Activin pathways in the pituitary. Expand


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A cDNA encoding the human thyrotropin-releasing hormone receptor (hTRH-R) was isolated from a human brain cDNA library. Screening of 1.2 million clones resulted in 2 candidates. The largest cloneExpand
Genomic Organization and Promoter Function of the Human Thyrotropin-releasing Hormone Receptor Gene*
Knowing the genomic organization and the promoter region of thyrotropin-releasing hormone (TRH) receptor gene will allow further studies of possible disorders of the TRH receptor, as well as facilitate elucidation of transcriptional control of the human TRH receptors gene. Expand
Two isoforms of the thyrotropin-releasing hormone receptor generated by alternative splicing have indistinguishable functional properties.
Functional expression in Xenopus laevis oocytes indicates that both cDNAs encode fully functional TRH receptors, and the perfect colinearity between genomic DNA and TRH-R(412) cDNA demonstrates that no other introns are present within the coding region of the TRH receptor gene. Expand
Molecular cloning and functional expression of a human thyrotropin-releasing hormone (TRH) receptor gene.
Results demonstrated that the isolated gene encoded a specific TRH receptor with high affinity, and showed high homology as a whole amino acid sequence with the rat and mouse TRH receptors except for considerable variation in the C-terminals. Expand
Structure of the luteinizing hormone receptor gene and multiple exons of the coding sequence.
The genomic structure of the LH receptor is important to our understanding of its expression mechanisms, functional domains, relationships with other hormone receptors, and evolution. We haveExpand
Expression cloning of a cDNA encoding the mouse pituitary thyrotropin-releasing hormone receptor.
Using Xenopus laevis oocytes in an expression cloning strategy, a cDNA clone is isolated that encodes the mouse pituitary TRH-R, a protein of 393 amino acids that shows similarities to other guanine nucleotide-binding regulatory protein-coupled receptors. Expand
Functional expression and molecular characterization of the thyrotrophin-releasing hormone receptor from the rat anterior pituitary gland.
The rat TRH-R protein showed considerable homology with that of the mouse, except for a deletion of 232 bp in the 3'-coding region, which did not appear to affect the functional characteristics of the receptor, as shown by electrophysiological studies with Xenopus oocytes and by transfection of the cDNA into COS-7 cells. Expand
Cloning and characterization of the chicken thyrotropin-releasing hormone receptor.
These findings show that there have been considerable evolutionary constraints on TRH receptor structure and function and provide a useful tool for further studies on the roles of TRH in avian growth and TSH regulation. Expand
Characterization of the mouse thyrotrophin-releasing hormone receptor gene: an exon corresponds to a deletion in the rat cDNA.
The cloning and characterization of the mouse TRH receptor (TRH-R) gene revealed an untranslated exon (exon 1), a single intron and an upstream dinucleotide repeat sequence (d(TG)16.d(AG)21) in theExpand
Molecular cloning of bovine thyrotropin-releasing hormone receptor gene.
RT-PCR analysis indicated TRHR mRNA to be expressed in the pituitary and brain and the deduced amino acid sequence of bovine TRHR exceeded 88% that of mouse, rat or human. Expand