Structural analysis of the yeast SWI/SNF chromatin remodeling complex

@article{Smith2003StructuralAO,
  title={Structural analysis of the yeast SWI/SNF chromatin remodeling complex},
  author={Corey L. Smith and Rachel A. Horowitz-Scherer and Joan Frances Flanagan and Christopher L. F. Woodcock and Craig L. Peterson},
  journal={Nature Structural Biology},
  year={2003},
  volume={10},
  pages={141-145}
}
Elucidating the mechanism of ATP-dependent chromatin remodeling is one of the largest challenges in the field of gene regulation. One of the missing pieces in understanding this process is detailed structural information on the enzymes that catalyze the remodeling reactions. Here we use a combination of subunit radio-iodination and scanning transmission electron microscopy to determine the subunit stoichiometry and native molecular weight of the yeast SWI/SNF complex. We also report a three… 
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173 Citations

Structure and function of SWI/SNF chromatin remodeling complexes and mechanistic implications for transcription.
Recent advances in understanding chromatin remodeling by Swi/Snf complexes.
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Multiple functions of SWI/SNF chromatin remodeling complex in plant-pathogen interactions
TLDR
Multiple functions of the SWI/SNF complex in eukaryotes are discussed in regulating gene transcription, mRNA splicing, and DNA damage response and the potentials in exploiting chromatin remodeling for management of crop disease are presented.
SWI/SNF Chromatin-remodeling Factors: Multiscale Analyses and Diverse Functions*
TLDR
The role of SWI/SNF in human diseases, including cancer and viral infections is addressed; recent genomic and proteomic advances are highlighted; and the biochemistry of these complex enzymes is reviewed.
Mechanism(s) of SWI/SNF‐Induced Nucleosome Mobilization
Impediments to DNA access due to assembly of the eukaryotic genome into chromatin are in part overcome by the activity of ATP‐dependent chromatin‐remodeling complexes. These complexes employ energy…
Structural analyses of the chromatin remodeling enzymes INO80-C and SWR-C
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EM studies define the position of the Arp8/Arp4/Act1 module within INO80-C, and it is found that this module enhances nucleosome binding affinity but is largely dispensable for remodeling activities.
Structural studies of the human PBAF chromatin-remodeling complex.
Studies on the mechanism of nucleosome remodeling by RSC and SWI/SNF
TLDR
It is demonstrated that the docking domain of histone H2A is essential for SWI/SNF and RSC induced nucleosome sliding and the reason for inability of these nucleosomes to slide is due to a faulty generation of ‘Remosome' intermediates.
Structural biochemistry of the INO80 chromatin remodeler reveals an unexpected function of its two subunits Arp4 and Arp8
TLDR
The goal of this study was to elucidate the functional architecture of the INO80 complex by using a hybrid methods approach and it is shown that Arp4 is able to inhibit actin polymerization and to depolymerize actin filaments most likely by complex formation with monomeric ADP-actin via the barbed end.
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