Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy.

@article{Ami2006StructuralAO,
  title={Structural analysis of protein inclusion bodies by Fourier transform infrared microspectroscopy.},
  author={Diletta Ami and Antonino Natalello and Geoffrey Taylor and Giancarlo Tonon and Silvia Maria Doglia},
  journal={Biochimica et biophysica acta},
  year={2006},
  volume={1764 4},
  pages={793-9}
}
The expression of recombinant human growth hormone (h-GH) and human interferon-alpha-2b (IFN-alpha-2b) in E. coli leads to the formation of insoluble protein aggregates or inclusion bodies (IBs). The secondary structure of these IBs, their corresponding native forms and thermal aggregates were studied by Fourier Transform Infrared (FT-IR) spectroscopy and microspectroscopy. It was demonstrated that residual native-like structures were maintained within IBs at different extents depending on the… CONTINUE READING

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