Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces.

Abstract

pSAM2 is an 11-kb plasmid integrated in the Streptomyces ambofaciens ATCC23877 and ATCC15154 genomes and found additionally as a free replicon in an uv derivative. After transfer into S. ambofaciens DSM40697 (devoid of pSAM2) or into Streptomyces lividans, specific integration of pSAM2 occurred very efficiently. A 58-bp sequence (att) present in both pSAM2… (More)

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@article{Boccard1989StructuralAO, title={Structural analysis of loci involved in pSAM2 site-specific integration in Streptomyces.}, author={Fr{\'e}d{\'e}ric Boccard and Tamara Smokvina and J. L. Pernodet and A. Friedmann and Marc Guerineau}, journal={Plasmid}, year={1989}, volume={21 1}, pages={59-70} }