Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy.

@article{Eskandari1998StructuralAO,
  title={Structural analysis of cloned plasma membrane proteins by freeze-fracture electron microscopy.},
  author={Sepehr Eskandari and Ernest M Wright and Michael Kreman and Dorine M. Starace and Guido A. Zampighi},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 19},
  pages={11235-40}
}
We have used freeze-fracture electron microscopy to examine the oligomeric structure and molecular asymmetry of integral plasma membrane proteins. Recombinant plasma membrane proteins were functionally expressed in Xenopus laevis oocytes, and the dimensions of their freeze-fracture particles were analyzed. To characterize the freeze-fracture particles, we compared the particle cross-sectional area of proteins with alpha-helical transmembrane domains (opsin, aquaporin 1, and a connexin) with… CONTINUE READING

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Proc. Natl. Acad. Sci. USA 95 • 1998

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