Structural analysis of a bioactive polysaccharide, PISP1, from the medicinal mushroom Phellinus igniarius.

@article{Yang2009StructuralAO,
  title={Structural analysis of a bioactive polysaccharide, PISP1, from the medicinal mushroom Phellinus igniarius.},
  author={Yan Yang and Libin Ye and Jingsong Zhang and Yan-Fang Liu and Qingjiu Tang},
  journal={Bioscience, biotechnology, and biochemistry},
  year={2009},
  volume={73 1},
  pages={134-9}
}
PISP1, a heteropolysaccharide isolated from fruiting bodies of Phellinus igniarius by hot aqueous extraction and purified by DEAE-Sepharose anion-exchange and gel filtration chromatography, is composed of fucose, galactose, mannose, and 3-O-Me-galactose in a ratio of 1:2:1:2. Methylation, monosaccharide analysis, and NMR studies (1H NMR, 13C NMR, COSY-45 degrees , TOCSY, ROESY, HSQC, and HMBC) revealed that PISP1 had a backbone consisting of 1,6-disubstituted-3-O-Me-alpha-D-galactopyranosyl… CONTINUE READING