Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle.

@article{Nishizawa2008StructuralAO,
  title={Structural analysis of 2D crystals of gastric H+,K+-ATPase in different states of the transport cycle.},
  author={Tomohiro Nishizawa and Kazuhiro Abe and Kazutoshi Tani and Yoshinori Fujiyoshi},
  journal={Journal of structural biology},
  year={2008},
  volume={162 2},
  pages={
          219-28
        }
}
The H+,K+-ATPase uses ATP to pump protons across the gastric membrane. We used electron crystallography and limited trypsin proteolysis to study conformational changes in the H+,K+-ATPase. Well-ordered 2D crystals were obtained with detergent-solubilized H+,K+-ATPase at low pH in the absence of nucleotides, E1 state, and in the presence of fluoroaluminate and ADP, mimicking the E1PADP state. Projection maps obtained with frozen-hydrated two-dimensional crystals of the H+,K+-ATPase in these two… CONTINUE READING