Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.

@article{Anders2016StructuralPO,
  title={Structural Plasticity of PAM Recognition by Engineered Variants of the RNA-Guided Endonuclease Cas9.},
  author={Carolin Anders and Katja Bargsten and Martin Jinek},
  journal={Molecular cell},
  year={2016},
  volume={61 6},
  pages={895-902}
}
The RNA-guided endonuclease Cas9 from Streptococcus pyogenes (SpCas9) forms the core of a powerful genome editing technology. DNA cleavage by SpCas9 is dependent on the presence of a 5'-NGG-3' protospacer adjacent motif (PAM) in the target DNA, restricting the choice of targetable sequences. To address this limitation, artificial SpCas9 variants with altered PAM specificities have recently been developed. Here we report crystal structures of the VQR, EQR, and VRER SpCas9 variants bound to… CONTINUE READING
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