Structural Basis of hAT Transposon End Recognition by Hermes, an Octameric DNA Transposase from Musca domestica

@article{Hickman2014StructuralBO,
  title={Structural Basis of hAT Transposon End Recognition by Hermes, an Octameric DNA Transposase from Musca domestica},
  author={Alison Burgess Hickman and Hosam E. Ewis and Xianghong Li and Joshua Knapp and T Laver and Anna-Louise Doss and G{\"o}khan Tolun and Alasdair C. Steven and Alexander Grishaev and Adriaan Bax and Peter W. Atkinson and Nancy L. Craig and Fred Dyda},
  journal={Cell},
  year={2014},
  volume={158},
  pages={353-367}
}
Hermes is a member of the hAT transposon superfamily that has active representatives, including McClintock's archetypal Ac mobile genetic element, in many eukaryotic species. The crystal structure of the Hermes transposase-DNA complex reveals that Hermes forms an octameric ring organized as a tetramer of dimers. Although isolated dimers are active in vitro for all the chemical steps of transposition, only octamers are active in vivo. The octamer can provide not only multiple specific DNA… CONTINUE READING