Structural Basis of Transcription Nucleotide Selection by Rotation in the RNA Polymerase II Active Center

@article{Westover2004StructuralBO,
  title={Structural Basis of Transcription Nucleotide Selection by Rotation in the RNA Polymerase II Active Center},
  author={Kenneth D. Westover and David A. Bushnell and Roger D. Kornberg},
  journal={Cell},
  year={2004},
  volume={119},
  pages={481-489}
}
Binding of a ribonucleoside triphosphate to an RNA polymerase II transcribing complex, with base pairing to the template DNA, was revealed by X-ray crystallography. Binding of a mismatched nucleoside triphosphate was also detected, but in an adjacent site, inverted with respect to the correctly paired nucleotide. The results are consistent with a two-step mechanism of nucleotide selection, with initial binding to an entry (E) site beneath the active center in an inverted orientation, followed… Expand
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