Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 Å Resolution

@article{Gnatt2001StructuralBO,
  title={Structural Basis of Transcription: An RNA Polymerase II Elongation Complex at 3.3 {\AA} Resolution},
  author={Averell L. Gnatt and Patrick Cramer and J C Fu and David A. Bushnell and Roger D. Kornberg},
  journal={Science},
  year={2001},
  volume={292},
  pages={1876 - 1882}
}
The crystal structure of RNA polymerase II in the act of transcription was determined at 3.3 Å resolution. Duplex DNA is seen entering the main cleft of the enzyme and unwinding before the active site. Nine base pairs of DNA-RNA hybrid extend from the active center at nearly right angles to the entering DNA, with the 3′ end of the RNA in the nucleotide addition site. The 3′ end is positioned above a pore, through which nucleotides may enter and through which RNA may be extruded during back… 
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