Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code

@article{Oshikane2006StructuralBO,
  title={Structural Basis of RNA-Dependent Recruitment of Glutamine to the Genetic Code},
  author={H. Oshikane and K. Sheppard and S. Fukai and Yuko Nakamura and R. Ishitani and T. Numata and R. L. Sherrer and L. Feng and E. Schmitt and M. Panvert and S. Blanquet and Y. Mechulam and D. S{\"o}ll and O. Nureki},
  journal={Science},
  year={2006},
  volume={312},
  pages={1950 - 1954}
}
Glutaminyl–transfer RNA (Gln-tRNAGln) in archaea is synthesized in a pretranslational amidation of misacylated Glu-tRNAGln by the heterodimeric Glu-tRNAGln amidotransferase GatDE. Here we report the crystal structure of the Methanothermobacter thermautotrophicus GatDE complexed to tRNAGln at 3.15 angstroms resolution. Biochemical analysis of GatDE and of tRNAGln mutants characterized the catalytic centers for the enzyme's three reactions (glutaminase, kinase, and amidotransferase activity). A… Expand
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