Structural Analysis of Protein-RNA Complexes in Solution Using NMR Paramagnetic Relaxation Enhancements.

@article{Hennig2015StructuralAO,
  title={Structural Analysis of Protein-RNA Complexes in Solution Using NMR Paramagnetic Relaxation Enhancements.},
  author={Janosch Hennig and Lisa Rose Warner and Bernd Simon and Arie Geerlof and Cameron David Mackereth and Michael Sattler},
  journal={Methods in enzymology},
  year={2015},
  volume={558},
  pages={
          333-362
        }
}
Biological activity in the cell is predominantly mediated by large multiprotein and protein-nucleic acid complexes that act together to ensure functional fidelity. Nuclear magnetic resonance (NMR) spectroscopy is the only method that can provide information for high-resolution three-dimensional structures and the conformational dynamics of these complexes in solution. Mapping of binding interfaces and molecular interactions along with the characterization of conformational dynamics is possible… CONTINUE READING