Stromal protein phosphorylation in spinach (Spinacia oleracea) chloroplasts.

  title={Stromal protein phosphorylation in spinach (Spinacia oleracea) chloroplasts.},
  author={Christine Helen Foyer},
  journal={The Biochemical journal},
  volume={231 1},
When intact spinach chloroplasts were supplied with [32P]Pi, stromal protein phosphorylation was found to occur in the dark. On illumination the thylakoid protein kinase was activated and the amount of label found in thylakoid proteins quickly exceeded that incorporated into stromal protein, such that the latter was found to account for only 10-15% of the total radioactivity bound to chloroplast proteins after 5 min illumination. The rate of phosphorylation of stromal polypeptides was unchanged… CONTINUE READING


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