Stringent doxycycline-dependent control of gene activities using an episomal one-vector system

@article{Bornkamm2005StringentDC,
  title={Stringent doxycycline-dependent control of gene activities using an episomal one-vector system},
  author={Georg W. Bornkamm and Christian Berens and Conny Kuklik-Roos and J M B{\'e}chet and Gerhard Laux and J{\"u}rgen Bachl and Martin Korndoerfer and Martin Schlee and Michael H{\"o}lzel and Anastassia Malamoussi and Rob D. Chapman and Falk Nimmerjahn and Josef Mautner and Wolfgang Hillen and Hermann Bujard and Jean Feuillard},
  journal={Nucleic Acids Research},
  year={2005},
  volume={33},
  pages={e137 - e137}
}
Conditional expression systems are of pivotal importance for the dissection of complex biological phenomena. Here, we describe a novel EBV-derived episomally replicating plasmid (pRTS-1) that carries all the elements for conditional expression of a gene of interest via Tet regulation. The vector is characterized by (i) low background activity, (ii) high inducibility in the presence of doxycycline (Dox) and (iii) graded response to increasing concentrations of the inducer. The chicken beta actin… CONTINUE READING
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