Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer.

@article{Jandle2006StringentAR,
  title={Stringent and relaxed recognition of oriT by related systems for plasmid mobilization: implications for horizontal gene transfer.},
  author={Sarah Jandle and Ralph Lawrence Meyer},
  journal={Journal of bacteriology},
  year={2006},
  volume={188 2},
  pages={
          499-506
        }
}
The plasmids R1162 and pSC101 have origins of conjugative transfer (oriTs) and corresponding relaxases that are closely related. The oriTs are made up of a highly conserved core, where DNA is cleaved by the relaxase prior to transfer, and an inverted repeat that differs in size and sequence. We show that in each case the seven base pairs adjacent to the core and within one arm of the inverted repeat are sufficient to determine specificity. Within this DNA there are three AT base pairs located 4… CONTINUE READING

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