Stretch-induced increases in intracellular calcium of isolated vascular smooth muscle cells.

@article{Davis1992StretchinducedII,
  title={Stretch-induced increases in intracellular calcium of isolated vascular smooth muscle cells.},
  author={Michael J Davis and Gerald A. Meininger and David C Zawieja},
  journal={The American journal of physiology},
  year={1992},
  volume={263 4 Pt 2},
  pages={H1292-9}
}
Vascular smooth muscle responds to stretch with an increase in active force development. To investigate the role of Ca2+ in this response, we used the fluorescent dye fura-2 to quantitate changes in cytosolic Ca2+ in single, vascular smooth muscle cells during rapid stretch. Cells were enzymatically dispersed from pig coronary arteries, loaded with fura-2/AM, and studied using a digital-imaging microscope. Stretch of individual cells was accomplished by attachment with suction to two patch-type… CONTINUE READING
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