Streptokinase: cloning, expression, and excretion by Escherichia coli.

  title={Streptokinase: cloning, expression, and excretion by Escherichia coli.},
  author={Horst Malke and Joseph J. Ferretti},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={81 11},
Genomic DNA from Streptococcus equisimilis strain H46A was cloned in Escherichia coli by using the bacteriophage lambda replacement vector L47 and an in vitro packaging system. A casein/plasminogen overlay technique was used to screen the phage bank for recombinants carrying the streptokinase gene ( skc ). The gene was present with a frequency of 1 in 836 recombinants, and 10 independent clones containing skc were isolated and physically characterized. One recombinant clone was used to subclone… CONTINUE READING


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