Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.

@article{Mller2016StreptococcusTC,
  title={Streptococcus thermophilus CRISPR-Cas9 Systems Enable Specific Editing of the Human Genome.},
  author={Maximilian M{\"u}ller and Ciaran M Lee and Giedrius Gasiūnas and Timothy H. Davis and Thomas J. Cradick and Virginijus Siksnys and Gang Bao and Toni Cathomen and Claudio Mussolino},
  journal={Molecular therapy : the journal of the American Society of Gene Therapy},
  year={2016},
  volume={24 3},
  pages={
          636-44
        }
}
RNA-guided nucleases (RGNs) based on the type II CRISPR-Cas9 system of Streptococcus pyogenes (Sp) have been widely used for genome editing in experimental models. However, the nontrivial level of off-target activity reported in several human cells may hamper clinical translation. RGN specificity depends on both the guide RNA (gRNA) and the protospacer adjacent motif (PAM) recognized by the Cas9 protein. We hypothesized that more stringent PAM requirements reduce the occurrence of off-target… CONTINUE READING
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