Streptococcus mutans genes that code for extracellular proteins in Escherichia coli K-12.

@article{Holt1982StreptococcusMG,
  title={Streptococcus mutans genes that code for extracellular proteins in Escherichia coli K-12.},
  author={Robert G Holt and Yoshimitsu Abiko and Shin-ya Saito and Maria Smorawinska and Jeffrey B. Hansen and Roy Curtiss},
  journal={Infection and immunity},
  year={1982},
  volume={38 1},
  pages={
          147-56
        }
}
Chromosomal DNA from Streptococcus mutans 6715 (serotype g) was cloned into Escherichia coli K-12 by using the cosmid pJC74 cloning vector and a bacteriophage lambda in vitro packaging system. Rabbit antiserum against S. mutans extracellular proteins was used for immunological screening of the clone bank. Twenty-one clones produced weak to strong precipitin bands around the colonies, but only after the lambda c1857 prophage was induced by being heated to lyse the E. coli cells. None of the… CONTINUE READING

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