Streptococcus mutans biofilm formation: utilization of a gtfB promoter-green fluorescent protein (PgtfB::gfp) construct to monitor development.

@article{Yoshida2002StreptococcusMB,
  title={Streptococcus mutans biofilm formation: utilization of a gtfB promoter-green fluorescent protein (PgtfB::gfp) construct to monitor development.},
  author={Akihiro Yoshida and Howard K. Kuramitsu},
  journal={Microbiology},
  year={2002},
  volume={148 Pt 11},
  pages={
          3385-94
        }
}
The glucosyltransferases of Streptococcus mutans are recognized as important virulence factors for this cariogenic bacterium. To study the expression of the gtfB gene of S. mutans in biofilms, a gtfB promoter (PgtfB)-green fluorescent protein (GFP) reporter system was developed. A Streptococcus-Escherichia coli shuttle vector harbouring a PGTFB::gfp cassette was introduced into S. mutans GS-5, and the expression of GFP by the transformed S. mutans cells was confirmed by fluorescence microscopy… 
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TLDR
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The findings suggest that mechanisms controlling virulence gene expression are variable among genotypes, providing the notion that the genetic diversity of S. mutans may have important implications for understanding mechanisms that regulate the expression of virulence genes in this species.
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References

SHOWING 1-10 OF 30 REFERENCES
Analysis of Gene Expression in Streptococcus Mutans in Biofilms in Vitro
TLDR
It is demonstrated that the polysaccharide synthesis machinery of S. mutans is differentially expressed in biofilms, and opens the way for a more detailed analysis of the environmental signals and signal transduction pathways governing the regulation of gene expression by S. Mutans cells that are immobilized on a solid surface.
Regulation of expression of Streptococcus mutans genes important to virulence
TLDR
Studies were initiated to investigate the regulation of Streptococcus mutans genes which are believed to be important to virulence and found that expression of these genes is increased by the presence of sucrose and is followed by a rapid decline in expression over time.
Quantitative detection of Streptococcus pneumoniae cells harbouring single or multiple copies of the gene encoding the green fluorescent protein.
A modified gfp gene from Aequorea victoria, encoding a variant of the green fluorescent protein (GFP), was subcloned into the mobilizable plasmid pMV158. gfp was placed under the control of the…
Green fluorescent protein as a novel species-specific marker in enteric dual-species biofilms.
TLDR
The methods described provide a convenient technique for the examination of mixed-species biofilm communities where the unique interactions between species determine the true properties of the resultant biofilms.
Isolation and characterization of the Streptococcus mutans gtfD gene, coding for primer-dependent soluble glucan synthesis.
TLDR
Two glucosyltransferase genes from Streptococcus mutans GS-5, gtfB and gtfC, have been previously isolated and sequenced in this laboratory, and a third gtf gene, gTFD, was isolated and characterized, indicating that three distinct gtf genes involved in glucan formation are present on the S. mutansGS-5 chromosome.
Assessment of GFP fluorescence in cells of Streptococcus gordonii under conditions of low pH and low oxygen concentration.
TLDR
GFP can be applied to studies of oxygen-tolerant anaerobic bacteria, and it is shown that densely packed, flowcell-grown biofilms of S. gordonii do not develop oxygen gradients inhibitory to GFP fluorescence development, and that the often transient nature of G FP fluorescence in acid-producing bacteria can be overcome in flowcells.
Construction of a model secretion system for oral streptococci
TLDR
Results suggest that in contrast to S. gordonii, the inability of S. mutans and S. milleri to secrete hybrid amylase molecules could result from incorrect interaction of the secretory components of these organisms with amyl enzyme precursor molecules.
Cloning of a Streptococcus mutans glucosyltransferase gene coding for insoluble glucan synthesis
TLDR
Results indicate that the gtfB gene codes for a GTF involved in insoluble glucan synthesis in strain GS-5, and evidence for the existence of a distinct gene sharing partial homology with gTFB was obtained.
Role of the Streptococcus mutans gtf genes in caries induction in the specific-pathogen-free rat model.
TLDR
The role of each of the Streptococcus mutans gtf genes coding for glucan synthesis in cariogenesis was evaluated by using strain UA130 in the specific-pathogen-free (SPF) rat model system, and results suggest that all four genes are important for smooth-surface caries formation.
Isolation and characterization of the Streptococcus mutans gtfC gene, coding for synthesis of both soluble and insoluble glucans
TLDR
Evidence for the presence of a third gtf gene coding for soluble glucan synthesis was obtained following the construction of mutants containing deletions of both the gtfB and gtfC genes.
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