Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genes.

@article{Dao1985StreptococcusEscherichiaCS,
  title={Streptococcus-Escherichia coli shuttle vector pSA3 and its use in the cloning of streptococcal genes.},
  author={My Lien Dao and Joseph J. Ferretti},
  journal={Applied and environmental microbiology},
  year={1985},
  volume={49 1},
  pages={115-9}
}
A shuttle vector that can replicate in both Streptococcus spp. and Escherichia coli has been constructed by joining the E. coli plasmid pACYC184 (chloramphenicol and tetracycline resistance) to the streptococcal plasmid pGB305 (erythromycin resistance). The resulting chimeric plasmid is designated pSA3 (chloramphenicol, erythromycin, and tetracycline resistance) and has seven unique restriction sites: EcoRI, EcoRV, BamHI, SalI, XbaI, NruI, and SphI. Molecular cloning into the EcoRI or EcoRV… CONTINUE READING