Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.

@article{Gibson1984StrategyFT,
  title={Strategy for the mass spectrometric verification and correction of the primary structures of proteins deduced from their DNA sequences.},
  author={Bradford W. Gibson and Klaus Biemann},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1984},
  volume={81 7},
  pages={1956-60}
}
Fast atom bombardment mass spectrometry has been used to confirm and correct regions from the amino acid sequences of three large proteins, glutaminyl- and glycyl-tRNA synthetase from Escherichia coli and methionyl-tRNA synthetase from yeast, whose primary structures had been deduced from the base sequences of their corresponding genes. The strategy is based on a comparison of the molecular weights of the tryptic peptides predicted from all three reading frames of the gene sequences with those… CONTINUE READING