Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system.

@article{Ko2013StrategyFS,
  title={Strategy for screening metagenomic resources for exocellulase activity using a robotic, high-throughput screening system.},
  author={Kyong-Cheol Ko and Yunjon Han and Dae-Eun Cheong and Jong Hyun Choi and Jae Jun Song},
  journal={Journal of microbiological methods},
  year={2013},
  volume={94 3},
  pages={311-6}
}
Exocellulases play a key role in cleaving the accessible ends of cellulose molecules to release soluble glucose and cellobiose. To date, there have been no screens for exocellulase owing to assay protocol limitations, the high cost of substrates, and low activity of exocellulases compared with endocellulases. This study is the first to demonstrate direct screening for exocellulase activity using a robotic, high-throughput screening (HTS) system. Cell growth in 96-well plates was measured by… CONTINUE READING

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