Strategy for membrane protein crystallization exemplified with OmpA and OmpX.

Abstract

The bacterial outer membrane proteins OmpA and OmpX were modified in such a manner that they yielded bulky crystals diffracting X-rays isotropically beyond 2 A resolution and permitting detailed structural analyses. The procedure involved semi-directed mutagenesis, mass production into inclusion bodies, and (re)naturation therefrom; it should be applicable… (More)

Topics

Cite this paper

@article{Pautsch1999StrategyFM, title={Strategy for membrane protein crystallization exemplified with OmpA and OmpX.}, author={Alex Pautsch and Josef A Vogt and Kirstin Model and Christian Siebold and Gabriele Schulz}, journal={Proteins}, year={1999}, volume={34 2}, pages={167-72} }