Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.

  title={Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.},
  author={Thomas Weissensteiner and Jerry S. S. Lanchbury},
  volume={21 6},
The use of the PCR method for routine testing has increased dramatically during recent years. Most assays involve co-amplification either of an internal control, of several alleles at a given locus or of a variety of bands produced by low-stringency primer annealing. In such multiplex reactions, certain products will often amplify preferentially. Amplimers that are more sensitive can be outcompeted under suboptimal PCR conditions, leading to assignment of false negatives. Optimization of PCR… CONTINUE READING
45 Citations
14 References
Similar Papers


Publications citing this paper.
Showing 1-10 of 45 extracted citations


Publications referenced by this paper.
Showing 1-10 of 14 references

DNA Enzymology above 100 ° C : topoisomerase V unlinks circular DNA at 80 – 119 ° C

  • D. E. Kellog, N. McKinney, D. Spasic, L. Goda, C. Levenson
  • Biol . Chem . Hoppe Seyler
  • 1995

Enhancement of HLA class II PCR - SSP by addition of cresol red and sucrose to the amplification mixtures

  • R. Higuchi
  • 1994

Investigation of methods for eliminating preferential amplification of alleles using DPB 1 as a model system

  • L. N. Johnston-Dow, M. D. McGinnis, M. P. Conrad, M. N. Kronick
  • Hum . Immunol .
  • 1994

Mistyping ACE heterozygotes

  • G Steeger
  • PCR Methods Appl .
  • 1993

Similar Papers

Loading similar papers…