Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.

@article{Weissensteiner1996StrategyFC,
  title={Strategy for controlling preferential amplification and avoiding false negatives in PCR typing.},
  author={Thomas Weissensteiner and Jerry S. S. Lanchbury},
  journal={BioTechniques},
  year={1996},
  volume={21 6},
  pages={
          1102-8
        }
}
The use of the PCR method for routine testing has increased dramatically during recent years. Most assays involve co-amplification either of an internal control, of several alleles at a given locus or of a variety of bands produced by low-stringency primer annealing. In such multiplex reactions, certain products will often amplify preferentially. Amplimers that are more sensitive can be outcompeted under suboptimal PCR conditions, leading to assignment of false negatives. Optimization of PCR… CONTINUE READING
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