Strategy for amplification and sequencing of insecticidal cry1A genes from Bacillus thuringiensis

@article{Sauka2006StrategyFA,
  title={Strategy for amplification and sequencing of insecticidal cry1A genes from Bacillus thuringiensis},
  author={Diego H. Sauka and Ariel Fernando Amadio and Rub{\'e}n O. Zandomeni and Graciela B. Benintende},
  journal={Antonie van Leeuwenhoek},
  year={2006},
  volume={91},
  pages={423-430}
}
A feasible and fully described strategy, with a detailed list of primers, for amplifying, cloning and sequencing known and potentially novel cry1A genes harboured by a Bacillus thuringiensis strain was successfully established. Based on the analysis of conserved regions of the cry1A genes, the 1AF and 1UR oligonucleotide primers were designed to amplify the whole open reading frame of these genes. The PCR products obtained revealed the successful amplification of cry1A genes from 13 B… CONTINUE READING

References

Publications referenced by this paper.
SHOWING 1-10 OF 24 REFERENCES

Bacillus thuringiensis and its pesticidal crystal proteins.

  • Microbiology and molecular biology reviews : MMBR
  • 1998
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