Strategies to maximize the performance of a STED microscope.

@article{Galiani2012StrategiesTM,
  title={Strategies to maximize the performance of a STED microscope.},
  author={Silvia Galiani and Benjamin Harke and Giuseppe Vicidomini and Gabriele Lignani and Fabio Benfenati and Alberto Diaspro and Paolo Bianchini},
  journal={Optics express},
  year={2012},
  volume={20 7},
  pages={7362-74}
}
In stimulated emission depletion (STED) microscopy, the spatial resolution scales as the inverse square root of the STED beam's intensity. However, to fully exploit the maximum effective resolution achievable for a given STED beam's intensity, several experimental precautions have to be considered. We focus our attention on the temporal alignment between… CONTINUE READING