Strategies for metabolic flux analysis in plants using isotope labelling.

  title={Strategies for metabolic flux analysis in plants using isotope labelling.},
  author={Adelbert Roscher and Nicholas J. Kruger and Robert Ratcliffe},
  journal={Journal of biotechnology},
  volume={77 1},
Flux measurements through metabolic pathways generate insights into the integration of metabolism, and there is increasing interest in using such measurements to quantify the metabolic effects of mutation and genetic manipulation. Isotope labelling provides a powerful approach for measuring metabolic fluxes, and it gives rise to several distinct methods based on either dynamic or steady-state experiments. We discuss the application of these methods to photosynthetic and non-photosynthetic plant… CONTINUE READING
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