Strategies for investigating the plant metabolic network with steady-state metabolic flux analysis: lessons from an Arabidopsis cell culture and other systems.

@article{Kruger2012StrategiesFI,
  title={Strategies for investigating the plant metabolic network with steady-state metabolic flux analysis: lessons from an Arabidopsis cell culture and other systems.},
  author={Nicholas J. Kruger and Shyam Kumar Masakapalli and R. George Ratcliffe},
  journal={Journal of experimental botany},
  year={2012},
  volume={63 6},
  pages={
          2309-23
        }
}
Steady-state (13)C metabolic flux analysis (MFA) is currently the experimental method of choice for generating flux maps of the compartmented network of primary metabolism in heterotrophic and mixotrophic plant tissues. While statistically robust protocols for the application of steady-state MFA to plant tissues have been developed by several research groups, the implementation of the method is still far from routine. The effort required to produce a flux map is more than justified by the… 

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