Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy.

@article{Hobot1991StrategiesFI,
  title={Strategies for improving the cytochemical and immunocytochemical sensitivity of ultrastructurally well-preserved, resin embedded biological tissue for light and electron microscopy.},
  author={Jan A. Hobot and Geoffrey R. Newman},
  journal={Scanning microscopy. Supplement},
  year={1991},
  volume={5 4},
  pages={S27-40; discussion S40-1}
}
Many techniques for processing tissue into resin are available, varying from conventional room temperature to low temperature procedures. The problem is to choose an appropriate method to suit the biological specimen under study. Room temperature approaches with aldehyde and osmium fixation do not give optimal retention of immunoreactivity. Osmium can be removed from sections, but recovery of immunosensitivity is reduced. Osmium post-fixation can be omitted, but heat polymerization of resins… CONTINUE READING