Strategies for cellular identification in nucleus tractus solitarius slices.

  title={Strategies for cellular identification in nucleus tractus solitarius slices.},
  author={Mark W Doyle and Timothy W Bailey and Y Jin and Suzanne M. Appleyard and Malcolm J Low and Michael C Andresen},
  journal={Journal of neuroscience methods},
  volume={137 1},
The indistinct regional anatomy and intermixing of second order neurons with projection and interneurons make cellular studies more difficult within the nucleus tractus solitarius (NTS). Here, we outline experimental strategies to join in vitro electrophysiological with neuroanatomical protocols to discriminate specific subpopulations of NTS neurons. Horizontally cutting the brain stem produces slices in which electrical activation of the solitary tract (ST) is free of local interneuron… CONTINUE READING
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