Strategic approach to produce low-cost, efficient, and stable competitive internal controls for detection of RNA viruses by use of reverse transcription-PCR.

@article{Villanova2007StrategicAT,
  title={Strategic approach to produce low-cost, efficient, and stable competitive internal controls for detection of RNA viruses by use of reverse transcription-PCR.},
  author={Gabriela Vanina Villanova and Daniela Gardiol and Miguel A. Taborda and Virginia Reggiardo and Hugo Tanno and Emilia D. Rivadeneira and Germ{\'a}n R P{\'e}rez and Adriana Angelica Giri},
  journal={Journal of clinical microbiology},
  year={2007},
  volume={45 11},
  pages={
          3555-63
        }
}
Molecular diagnostics based on reverse transcription (RT)-PCR are routinely complicated by the lack of stable internal controls, leading to falsely negative results. We describe a strategy to produce a stable competitive internal control (CIC) based on a Qbeta phage derivative (recombinant Qbeta [rQbeta]) bearing primers KY78 and KY80, which are widely used in the detection of hepatitis C virus (HCV). rQbeta was RNase resistant and stable at 4 degrees C for 452 days in SM medium (0.1 M NaCl, 8… CONTINUE READING

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