Strand-specificity in the transformation of yeast with synthetic oligonucleotides.

@article{Yamamoto1992StrandspecificityIT,
  title={Strand-specificity in the transformation of yeast with synthetic oligonucleotides.},
  author={Tadashi Yamamoto and Richard P. Moerschell and L P Wakem and S Komar-Panicucci and Fred Sherman},
  journal={Genetics},
  year={1992},
  volume={131 4},
  pages={811-9}
}
Cyc1 mutants of the yeast Saccharomyces cerevisiae were directly transformed with both sense and antisense oligonucleotides to examine the involvement of the two genomic DNA strands in transformation. Sense oligonucleotides yielded approximately 20-fold more transformants than antisense oligonucleotides. This differential effect was observed with oligonucleotides designed to make alterations at six different sites along the gene and was independent of the oligonucleotide sequence and length… CONTINUE READING

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