Strand-specific analysis shows protein binding at replication forks and PCNA unloading from lagging strands when forks stall.

@article{Yu2014StrandspecificAS,
  title={Strand-specific analysis shows protein binding at replication forks and PCNA unloading from lagging strands when forks stall.},
  author={Chuanhe Yu and Haiyun Gan and Junhong Han and Zhi-Xiong Zhou and Shaodong Jia and Andrei Chabes and Gianrico Farrugia and Tamas Ordog and Zhiguo Zhang},
  journal={Molecular cell},
  year={2014},
  volume={56 4},
  pages={551-63}
}
In eukaryotic cells, DNA replication proceeds with continuous synthesis of leading-strand DNA and discontinuous synthesis of lagging-strand DNA. Here we describe a method, eSPAN (enrichment and sequencing of protein-associated nascent DNA), which reveals the genome-wide association of proteins with leading and lagging strands of DNA replication forks. Using this approach in budding yeast, we confirm the strand specificities of DNA polymerases delta and epsilon and show that the PCNA clamp is… CONTINUE READING
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Strand-Specific Analysis Shows Protein Binding at Replication Forks and PCNA Unloading from Lagging Strands when Forks Stall

  • Yu
  • Molecular Cell (2014),
  • 2014
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