Strain improvement of Aspergillus niger for phytase production

@article{Chelius2004StrainIO,
  title={Strain improvement of Aspergillus niger for phytase production},
  author={Marisa K. Chelius and Rudy J. Wodzinski},
  journal={Applied Microbiology and Biotechnology},
  year={2004},
  volume={41},
  pages={79-83}
}
A strain improvement program was developed to increase extracellular phytase (E.C. 3.1.3.8.) production by Aspergillus niger (syn. A. ficuum) NRRL 3135. Ultraviolet (UV) radiation was used as the mutagen and resistance to 50 μg/ml of hygromycin B as the selection method. Mutant 2DE, the product of two UV treatments, had phytase (PhytA) activity at pH 5.0 in the extracellular filtrate that was 3.3-fold higher than the wild-type activity. The activity of the non-specific acid phosphatase with a… CONTINUE READING

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The mutant had an extracellular protein concentration 3.2-fold higher than the wild type , which correlated with its higher phytase activity at pH 5.0 , but not with phytase activity at pH 2.5 and acid phosphatase activities .
The mutant had an extracellular protein concentration 3.2-fold higher than the wild type , which correlated with its higher phytase activity at pH 5.0 , but not with phytase activity at pH 2.5 and acid phosphatase activities .
The mutant had an extracellular protein concentration 3.2-fold higher than the wild type , which correlated with its higher phytase activity at pH 5.0 , but not with phytase activity at pH 2.5 and acid phosphatase activities .
The mutant had an extracellular protein concentration 3.2-fold higher than the wild type , which correlated with its higher phytase activity at pH 5.0 , but not with phytase activity at pH 2.5 and acid phosphatase activities .
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